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AbiotiK+ Stress-Potassium transport in abiotic stress in plants and yeast
GENERAL DESCRIPTION OF SCIENTIFIC INTERESTS:
Ion homeostasis is a dynamic process and a fundamental requirement for all organisms. Many different minerals are required for essential biochemical processes, but accumulation of these elements is toxic. Thus, all living organisms have developed efficient systems to acquire and store these elements and robust mechanisms to maintain homeostatic concentrations to avoid toxicity and to respond to environmental changes. Potassium is a key monovalent cation necessary for multiple aspects of cell growth and survival, for example compensation of negative charges of macromolecules, maintenance of electroneutrality, cell turgor and volume, enzyme activity, protein synthesis, and maintenance of proper membrane potential and intracellular pH. The long term, general goal of our research group is to generate new knowledge regarding the regulation of potassium transporters from both plants and yeast which may be applied in future in biotechnological approaches to improve plant drought tolerance and industrial performance of yeast.
In plants, apart from the basic, general physiological functions listed above for potassium at the cellular level, this cation also plays a key role at the whole plant level, as it is involved in important processes such as stomatal aperture that controls transpirational water loss and plant desiccation. Inward rectifying channels (Kin) are responsible for potassium influx into guard cells and play a key role in stomatal opening. KAT1, and its close homologue KAT2, are the main inward rectifying channels expressed in guard cells. Our current project is focused on the characterization 14 proteins that we have identified in a Split-Ubiquitin protein-protein interaction screen searching for KAT1 potassium channel interactors from the model plant Arabidopsis thaliana. We are taking several biochemical and genetic approaches to confirm these interactors and their effect on KAT1 activity in plants. The identification of physiologically relevant regulators of K+ channels will aid in the design of approaches that may impact both drought tolerance and pathogen susceptibility, since these pores are responsible for CO2 uptake and transpirational water loss and are the point of entry for certain pathogens.
In addition we are interested in studying the regulation of the high affinity potassium transporter of yeast, Trk1. The regulation of this transporter is crucial for the regulation of nutrient uptake and, recently, a direct effect of both external and internal potassium and pH on ethanol tolerance in conditions relevant for the industrial production of bioethanol has been reported. In addition, several studies clearly indicate that the proteins involved in determining and maintaining plasma membrane potential through the modulation of potassium homeostasis represent promising targets for complimentary antifungal treatments. Therefore, the identification of proteins that regulate the activity of this potassium transporter has potential applications in both agronomic, industrial and medical contexts.
Arrillaga I, Gil-Mascarell R, Gisbert C, Sales E, Montesinos C, Serrano R, Moreno V (1998)Expression of the yeast HAL2 gene in tomato increases the in vitro salt tolerance of transgenic progenies
Plant Science 136: 219-226
De Nadal E, Clotet J, Posas F, Serrano R, Gomez N, Arino J (1998)The yeast halotolerance determinant Hal3p is an inhibitory subunit of the Ppz1p ser/Thr protein phosphatase
Proceedings of The National Academy of Sciences of The United States of America 95: 7357-7362
Mendizabal I, Rios G, Mulet JM, Serrano R, de Larrinoa IF (1998)Yeast putative transcription factors involved in salt tolerance
FEBS Letters 425: 323-328
Garcia MJ, Rios G, Ali R, Belles JM, Serrano R (1997)Comparative physiology of salt tolerance in candida tropicalis and saccharomyces cerevisiae
Microbiology-Uk 143: 1125-1131
Romero C, Belles JM, Vaya JL, Serrano R, CulianezMacia FA (1997)Expression of the yeast trehalose-6-phosphate synthase gene in transgenic tobacco plants: Pleiotropic phenotypes include drought tolerance
Planta 201: 293-297
Rios G, Ferrando A, Serrano R (1997)Mechanisms of salt tolerance conferred by overexpression of the hal1 gene in saccharomyces cerevisiae
Yeast 13: 515-528
Bordas, M, Montesinos, C, Dabauza, M, Salvador A, Roig LA, Serrano R, Moreno V (1997)Transfer of the yeast salt tolerance gene HAL1 to cucumis melo l cultivars and in vitro evaluation of salt tolerance
Transgenic Research 6: 41-50
Rodriguez PL, Ali R, Serrano R (1996)Ctcdc55p and ctha13p: two putative regulatory proteins from candida tropicalis with long acidic domains
Yeast 12: 1321-1329
Campos F, PerezCastineira JR Villalba JM, CulianezMarcia FA, Sanchez F, Serrano R (1996)Localization of plasma membrane h+-atpase in nodules of phaseolus vulgaris l.
Plant Molecular Biology 32: 1043-1053
Marquez JA, Serrano R (1996)Multiple transduction pathways regulate the sodium-extrusion gene PMR2/ENA1 during salt stress in yeast
FEBS Letters 382: 89-92