Grupos de investigación

Grupo: Estrés AbiótiKo-Transporte de potasio en estrés abiótico en plantas y levaduras

AbiotiK+ Stress-Potassium transport in abiotic stress in plants and yeast

GENERAL DESCRIPTION OF SCIENTIFIC INTERESTS:
Ion homeostasis is a dynamic process and a fundamental requirement for all organisms. Many different minerals are required for essential biochemical processes, but accumulation of these elements is toxic. Thus, all living organisms have developed efficient systems to acquire and store these elements and robust mechanisms to maintain homeostatic concentrations to avoid toxicity and to respond to environmental changes. Potassium is a key monovalent cation necessary for multiple aspects of cell growth and survival, for example compensation of negative charges of macromolecules, maintenance of electroneutrality, cell turgor and volume, enzyme activity, protein synthesis, and maintenance of proper membrane potential and intracellular pH. The long term, general goal of our research group is to generate new knowledge regarding the regulation of potassium transporters from both plants and yeast which may be applied in future in biotechnological approaches to improve plant drought tolerance and industrial performance of yeast.

CURRENT PROJECTS:
In plants, apart from the basic, general physiological functions listed above for potassium at the cellular level, this cation also plays a key role at the whole plant level, as it is involved in important processes such as stomatal aperture that controls transpirational water loss and plant desiccation. Inward rectifying channels (Kin) are responsible for potassium influx into guard cells and play a key role in stomatal opening. KAT1, and its close homologue KAT2, are the main inward rectifying channels expressed in guard cells. Our current project is focused on the characterization 14 proteins that we have identified in a Split-Ubiquitin protein-protein interaction screen searching for KAT1 potassium channel interactors from the model plant Arabidopsis thaliana. We are taking several biochemical and genetic approaches to confirm these interactors and their effect on KAT1 activity in plants. The identification of physiologically relevant regulators of K+ channels will aid in the design of approaches that may impact both drought tolerance and pathogen susceptibility, since these pores are responsible for CO2 uptake and transpirational water loss and are the point of entry for certain pathogens.
In addition we are interested in studying the regulation of the high affinity potassium transporter of yeast, Trk1. The regulation of this transporter is crucial for the regulation of nutrient uptake and, recently, a direct effect of both external and internal potassium and pH on ethanol tolerance in conditions relevant for the industrial production of bioethanol has been reported. In addition, several studies clearly indicate that the proteins involved in determining and maintaining plasma membrane potential through the modulation of potassium homeostasis represent promising targets for complimentary antifungal treatments. Therefore, the identification of proteins that regulate the activity of this potassium transporter has potential applications in both agronomic, industrial and medical contexts.

Investigadores

Personal Contratado y Becarios

  • Alejandro S, Rodriguez PL, Bellés JM, Yenush L, Garcia-Sanchez MJ, Fernandez JA, Serrano R (2007)
    An Arabidopsis quiescin-sulfhydryl oxidase regulates cation homeostasis at the root symplast-xylem interface

    EMBO Journal 26: 3203-3215

  • Pérez-Valle J, Jenkins H, Merchan S, Montiel V, Ramos J, Sharma S, Serrano R, Yenush L (2007)
    Key role for intracellular K+ and protein kinases Sat4/Hal4 and Hal5 in the plasma membrane stabilization of yeast nutrient transporters

    Molecular and Cellular Biology 27: 5725-5736

  • Portillo F, Mulet JM, Serrano R (2005)
    A Role for the non-phosphorylated Form of Yeast Snf1: Tolerance to Toxic Cations and Activation of Potassium Transporter

    FEBS letters 579: 512-516

  • Yenush L, Merchan S, Holmes J, Serrano R (2005)
    pH-Responsive, posttranslational regulation of the Trk1 potassium transporter by the type 1-related Ppz1 phosphatase

    Molecular and Cellular Biology 25: 8683-8692

  • Mulet JM, Alemany B, Ros R, Calvete JJ, Serrano R (2004)
    Expression of a Plant Serine O-acetyltransferase in Saccharomyces cerevisiae Confers Osmotic Tolerance and Creates and Alternative Pathway for Cysteine Biosynthesis

    Yeast 21: 303-312

  • Merchan S, Bernal D, Serrano R, Yenush L (2004)
    Response of the yeast Mpk1 MAP kinase pathway to increases in internal turgor pressure caused by loss of the Ppz protein phosphatases

    Eukaryotic Cell 3: 100-107

  • Mulet JM, Alejandro S, Romero C, Serrano R (2004)
    The Trehalose Pathway and Intracellular Glucose Phosphates as Modulators of Potassium Transport and General Cation Homeostasis in Yeast

    Yeast 21: 569-582

  • Ruiz A, Yenush L, Ariño J (2003)
    Regulation of ENA1 Na(+)-ATPase Gene Expression by the Ppz1 Protein Phosphatase Is Mediated by the Calcineurin Pathway

    Eukaryotic Cell 2: 937-948

  • Rausell A, Kanhonou R, Yenush L, Serrano R, Ros R (2003)
    The translation initiation factor eIF1A is an important determinant in the tolerance to NaCl stress in yeast and plants

    Plant Journal 34: 1-11

  • Borrell A, Cutanda MC, Lumbreras V, Pujal J, Goday A, Culianez-Macia FA, Pages M (2002)
    Arabidopsis thaliana Atrab28: a nuclear targeted protein related to germination and toxic cation tolerance

    Plant Molecular Biology 50: 249-259

Introducción de genes relacionados con la tolerancia a estrés hídrico y oxidativo en distintos materiales que presentan características útiles para su uso como patrones de plantas hortícolas de interés agronómico (Nuevas kíneas de investigación multidisciplinares), 01-12-2010/30-11-2012.
Entidad financiadora: 
Universitat Politècnica de València
IP: 
Carmina Gisbert
Caracterización molecular del gen CRIO4 de remolacha y su aplicación en el desarrollo de líneas tolerantes a estrés por frío. (Primeros proyectos de investigación-1496), 01-11-2010/31-10-2012.
Entidad financiadora: 
Universitat Politècnica de València
IP: 
José Miguel Mulet
BFU2008-04188-C03-02. "Rutas de Transduccion de Señales en la Regulacion de la Homeostasis Ionica"
Entidad financiadora: 
Ministerio de Economía y Competitividad/FEDER
IP: 
Lynne Yenush
BFU2008-04188-C03-02. "Rutas de Transduccion de Señales en la Regulacion de la Homeostasis Ionica"
Entidad financiadora: 
MICINN/FEDER
IP: 
Lynne Yenush
DESCIFRANDO LA REGULACION DE TRANSPORTADORES DE POTASIO EN PLANTAS Y LEVADURAS (BIO2016-77776-P) 1/2017-12/2019
Entidad financiadora: 
MINISTERIO DE ECONOMÍA Y COMPETITIVIDAD/FEDER
IP: 
Lynne Yenush y JM Mulet
Título: 
METHOD TO INCREASE STRESS TOLERANCE IN PLANTS
Fecha de solicitud: 
Jueves, 21 Octubre, 2004
Referencia ID: 
A method is presented for selecting and isolating nucleic acids capable of conferring tolerance or resistance to environmental stress conditions in plants or yeast. Furthermore, nucleic acids, the proteins they encode and their use for the production of plants or yeast with enhanced environmental stress resistance is disclosed.
Autores: 
Serrano Salom, Ramón Mulet Salort, José Miguel
Título: 
PLANT HAEMOGLOBIN
Fecha de solicitud: 
Jueves, 14 Octubre, 2004
Referencia ID: 
The invention relates generally to a method for improving growth of plants, under normal and under stress conditions, more particularly under osmotic stress and/or temperature extremes, comprising modifying plant haemoglobin gene expression and/or by modifying plant haemoglobin protein levels in a plant. The invention furthermore relates to a method for increasing yield of a plant, comprising modifying plant gene expression and/or by modifying plant haemoglobin levels in a plant. The invention also relates to a nucleic acid encoding a haemoglobin conferring this altered growth and stress tolerance.
Autores: 
Mulet Salort, Jose Miguel Sanz Molinero, Ana Isabel Serrano Salom, Ramon
Título: 
(ARKL-CRIO5) Plants having enhanced yield-related traits and a method for making the same.
Fecha de solicitud: 
Lunes, 15 Septiembre, 2008
Autores: 
Valerie Frankard Cristophe Reuzeau Ana I. Sanz Molinero Ramon Serrano JM Mulet Salort
Título: 
(Patellin-CRIO4) Plants having enhanced yield-related traits and a method for making the same
Fecha de solicitud: 
Viernes, 28 Noviembre, 2008
Autores: 
Ana I. Sanz Molinero Cristophe Reuzeau Valerie Frankard Ramón Serrano Salom JM Mulet Salort
Yeast ion homeostasis