Grupos de investigación

Grupo: Estrés AbiótiKo-Transporte de potasio en estrés abiótico en plantas y levaduras

AbiotiK+ Stress-Potassium transport in abiotic stress in plants and yeast

GENERAL DESCRIPTION OF SCIENTIFIC INTERESTS:
Ion homeostasis is a dynamic process and a fundamental requirement for all organisms. Many different minerals are required for essential biochemical processes, but accumulation of these elements is toxic. Thus, all living organisms have developed efficient systems to acquire and store these elements and robust mechanisms to maintain homeostatic concentrations to avoid toxicity and to respond to environmental changes. Potassium is a key monovalent cation necessary for multiple aspects of cell growth and survival, for example compensation of negative charges of macromolecules, maintenance of electroneutrality, cell turgor and volume, enzyme activity, protein synthesis, and maintenance of proper membrane potential and intracellular pH. The long term, general goal of our research group is to generate new knowledge regarding the regulation of potassium transporters from both plants and yeast which may be applied in future in biotechnological approaches to improve plant drought tolerance and industrial performance of yeast.

CURRENT PROJECTS:
In plants, apart from the basic, general physiological functions listed above for potassium at the cellular level, this cation also plays a key role at the whole plant level, as it is involved in important processes such as stomatal aperture that controls transpirational water loss and plant desiccation. Inward rectifying channels (Kin) are responsible for potassium influx into guard cells and play a key role in stomatal opening. KAT1, and its close homologue KAT2, are the main inward rectifying channels expressed in guard cells. Our current project is focused on the characterization 14 proteins that we have identified in a Split-Ubiquitin protein-protein interaction screen searching for KAT1 potassium channel interactors from the model plant Arabidopsis thaliana. We are taking several biochemical and genetic approaches to confirm these interactors and their effect on KAT1 activity in plants. The identification of physiologically relevant regulators of K+ channels will aid in the design of approaches that may impact both drought tolerance and pathogen susceptibility, since these pores are responsible for CO2 uptake and transpirational water loss and are the point of entry for certain pathogens.
In addition we are interested in studying the regulation of the high affinity potassium transporter of yeast, Trk1. The regulation of this transporter is crucial for the regulation of nutrient uptake and, recently, a direct effect of both external and internal potassium and pH on ethanol tolerance in conditions relevant for the industrial production of bioethanol has been reported. In addition, several studies clearly indicate that the proteins involved in determining and maintaining plasma membrane potential through the modulation of potassium homeostasis represent promising targets for complimentary antifungal treatments. Therefore, the identification of proteins that regulate the activity of this potassium transporter has potential applications in both agronomic, industrial and medical contexts.

Investigadores

Personal Contratado y Becarios

  • Petrezselyova S, Lopez-Malo M, Canadell D, Roque A, Serra-Cardona A, Marquea MC, Vilaprinyo E, Alves R, Yenush L, Arino J (2016)
    Regulation of the Na+/K+-ATPase Ena1 Expression by Calcineurin/Crz1 under High pH Stress: A Quantitative Study
    Plos One 11: e0158424
  • Llopis-Torregrosa V, Ferri-Blazquez A, Adam-Artigues A, Deffontaines E, van Heusden GPH, Yenush L (2016)
    Regulation of the Yeast Hxt6 Hexose Transporter by the Rod1 alpha-Arrestin, the Snf1 Protein Kinase, and the Bmh2 14-3-3 Protein.
    The Journal of biological chemistry 291: 14973-14985
  • Marqués MC, Zamarbide-Forés S, Pedelini L, Llopis-Torregrosa V, Yenush L (2015)
    A functional Rim101 complex is required for proper accumulation of the Ena1 Na+-ATPase protein in response to salt stress in Saccharomyces cerevisiae

    FEMS Yeast Research 15: fov017

  • Mattiazzi Ušaj M, Prelec M, Brložnik M, Primo C, Curk T, Ščančar J, Yenush L, Petrovič U (2015)
    Yeast Saccharomyces cerevisiae adiponectin receptor homolog Izh2 is involved in the regulation of zinc, phospholipid and pH homeostasis

    Metallomics 7: 1338-1351

  • Bueso E, Muñoz-Bertomeu J, Campos F, Brunaud V, Martinez L, Sayas E, Ballester P, Yenush L, Serrano R (2014)
    ARABIDOPSIS THALIANA HOMEOBOX 25 uncovers a role for gibberellins in seed longevity

    Plant Physiology 164: 999-1010

  • Ariño J, Aydar E, Drulhe S, Ganser D, Jorrín J, Kahm M, Krause F, Petrezsélyová S, Yenush L, Zimmermannová O, van Heusden GP, Kschischo M, Ludwig J, Palmer C, Ramos J, Sychrová H (2014)
    Systems biology of monovalent cation homeostasis in yeast: the translucent contribution

    Advances in Microbial Physiology 64: 1-63

  • Mulet JM, Llopis-Torregrosa V, Primo C, Marqués MC, Yenush L (2013)
    Endocytic regulation of alkali metal transport proteins in mammals, yeast and plants

    Current Genetics 59: 207-230

  • Roque E, Serwatowska J, Cruz Rochina M, Wen J, Mysore KS, Yenush L, Beltrán JP, Cañas LA (2013)
    Functional specialization of duplicated AP3-like genes in Medicago truncatula

    Plant Journal 73: 663-675

  • Sainz M, Pérez-Rontomé C, Ramos J, Mulet JM, James EK, Bhattacharjee U, Petrich JW, Becana M (2013)
    Plant hemoglobins may be maintained in functional form by reduced flavins in the nuclei, and confer differential tolerance to nitro-oxidative stress

    Plant Journal 76: 875-887

  • Ríos G, Cabedo M, Rull B, Yenush L, Serrano R, Mulet JM (2013)
    Role of the yeast multidrug transporter Qdr2 in cation homeostasis and the oxidative stress response

    FEMS Yeast Research 13: 97-106

Entidad financiadora: 
Universitat Politècnica de València
IP: 
Carmina Gisbert
Entidad financiadora: 
Universitat Politècnica de València
IP: 
José Miguel Mulet
Entidad financiadora: 
Ministerio de Economía y Competitividad/FEDER
IP: 
Lynne Yenush
Entidad financiadora: 
MICINN/FEDER
IP: 
Lynne Yenush
Entidad financiadora: 
MINISTERIO DE ECONOMÍA Y COMPETITIVIDAD/FEDER
IP: 
Lynne Yenush y JM Mulet
Título: 
METHOD TO INCREASE STRESS TOLERANCE IN PLANTS
Fecha de solicitud: 
Jueves, 21 Octubre, 2004
Referencia ID: 
A method is presented for selecting and isolating nucleic acids capable of conferring tolerance or resistance to environmental stress conditions in plants or yeast. Furthermore, nucleic acids, the proteins they encode and their use for the production of plants or yeast with enhanced environmental stress resistance is disclosed.
Autores: 
Serrano Salom, Ramón Mulet Salort, José Miguel
Título: 
PLANT HAEMOGLOBIN
Fecha de solicitud: 
Jueves, 14 Octubre, 2004
Referencia ID: 
The invention relates generally to a method for improving growth of plants, under normal and under stress conditions, more particularly under osmotic stress and/or temperature extremes, comprising modifying plant haemoglobin gene expression and/or by modifying plant haemoglobin protein levels in a plant. The invention furthermore relates to a method for increasing yield of a plant, comprising modifying plant gene expression and/or by modifying plant haemoglobin levels in a plant. The invention also relates to a nucleic acid encoding a haemoglobin conferring this altered growth and stress tolerance.
Autores: 
Mulet Salort, Jose Miguel Sanz Molinero, Ana Isabel Serrano Salom, Ramon
Título: 
(ARKL-CRIO5) Plants having enhanced yield-related traits and a method for making the same.
Fecha de solicitud: 
Lunes, 15 Septiembre, 2008
Autores: 
Valerie Frankard Cristophe Reuzeau Ana I. Sanz Molinero Ramon Serrano JM Mulet Salort
Título: 
(Patellin-CRIO4) Plants having enhanced yield-related traits and a method for making the same
Fecha de solicitud: 
Viernes, 28 Noviembre, 2008
Autores: 
Ana I. Sanz Molinero Cristophe Reuzeau Valerie Frankard Ramón Serrano Salom JM Mulet Salort
Identificación y caracterización del gen Xer02 de remolacha (Beta vulgaris)
Año: 
2011
Nombre: 
Victor Castillo Ibañez
Universidad: 
Universitat Politècnica de València, ETSIAMN
Tipo: 
Trabajos de Fin de Carrera
Cultivo in vitro e inducción de la organogénesis en cultivares de bejenjena (Solanum melongena) y en la especie relacionada Solanum torvum. Micropropagación de introducción del gen BvSAT mediante transformación genética en S. Torvum.
Año: 
2011
Nombre: 
Perelló Pinazo, Adriá
Universidad: 
Universitat Politècnica de València
Dirigida por: 
Dr. Jose Miguel Mulet Salort y Dra. Carmen Gisbert Domenech.
Tipo: 
Trabajos de Fin de Carrera
Caracterización de la regulación de la protón-ATPasa Pma1 por parte de la ruta TOR en levadura.
Año: 
2010
Nombre: 
Marc Cabedo López
Universidad: 
Universitat Politècnica de València
Tipo: 
Trabajos de Fin de Carrera
Caracterización Fenotípica de Mutantes de Arabidopsis thaliana en los Genes de las Prolil Isomerasas Rof1 y Rof2.
Año: 
2010
Nombre: 
Sandra Fresquet Corrales
Universidad: 
Universitat Politècnica de València
Dirigida por: 
Mulet Salort, Jose Miguel y Serrano Salom, Ramón.
Tipo: 
Tesis de Master
Estudio de los mecanismos de la regulación de la homeostasis iónica: Análisis fisiológico y transcriptómico del mutante hal4 hal5 de Saccharomyces cerevisiae.
Año: 
2009
Nombre: 
Pérez-Valle, Jorge
Universidad: 
Universitat Politècnica de València
Dirigida por: 
Yenush, Lynne; Serrano, Ramón.
Tipo: 
Tesis Doctorales
Identificación y caracterización del gen Crio4 de remolacha (Beta vulgaris).
Año: 
2008
Nombre: 
Izquierdo García, Ana Cristina
Universidad: 
Universitat Politècnica de València
Dirigida por: 
Mulet Salort, Jose Miguel.
Tipo: 
Tesis de Master
Cellular implications of alterations in potassium homeostasis in Saccharomyces cerevisiae.
Año: 
2007
Nombre: 
Merchan, Stephanie
Universidad: 
Universitat Politècnica de València
Dirigida por: 
Yenush, Lynne; Serrano, Ramón
Tipo: 
Tesis Doctorales
Caracterizacion del sistema de transporte codificado por el gen de halotolerancia HAL11/QDR2 de Saccharomyces cerevisiae.
Nombre: 
Rull Costa, Baltasar
Universidad: 
Universitat Politècnica de València. Escuela Técnica Superior de Ingenieros Agrónomos.
Dirigida por: 
Mulet Salort, JM; Serrano Salom, Ramón
Tipo: 
Trabajos de Fin de Carrera
Yeast ion homeostasis