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AbiotiK+ Stress-Potassium transport in abiotic stress in plants and yeast
GENERAL DESCRIPTION OF SCIENTIFIC INTERESTS:
Ion homeostasis is a dynamic process and a fundamental requirement for all organisms. Many different minerals are required for essential biochemical processes, but accumulation of these elements is toxic. Thus, all living organisms have developed efficient systems to acquire and store these elements and robust mechanisms to maintain homeostatic concentrations to avoid toxicity and to respond to environmental changes. Potassium is a key monovalent cation necessary for multiple aspects of cell growth and survival, for example compensation of negative charges of macromolecules, maintenance of electroneutrality, cell turgor and volume, enzyme activity, protein synthesis, and maintenance of proper membrane potential and intracellular pH. The long term, general goal of our research group is to generate new knowledge regarding the regulation of potassium transporters from both plants and yeast which may be applied in future in biotechnological approaches to improve plant drought tolerance and industrial performance of yeast.
In plants, apart from the basic, general physiological functions listed above for potassium at the cellular level, this cation also plays a key role at the whole plant level, as it is involved in important processes such as stomatal aperture that controls transpirational water loss and plant desiccation. Inward rectifying channels (Kin) are responsible for potassium influx into guard cells and play a key role in stomatal opening. KAT1, and its close homologue KAT2, are the main inward rectifying channels expressed in guard cells. Our current project is focused on the characterization 14 proteins that we have identified in a Split-Ubiquitin protein-protein interaction screen searching for KAT1 potassium channel interactors from the model plant Arabidopsis thaliana. We are taking several biochemical and genetic approaches to confirm these interactors and their effect on KAT1 activity in plants. The identification of physiologically relevant regulators of K+ channels will aid in the design of approaches that may impact both drought tolerance and pathogen susceptibility, since these pores are responsible for CO2 uptake and transpirational water loss and are the point of entry for certain pathogens.
In addition we are interested in studying the regulation of the high affinity potassium transporter of yeast, Trk1. The regulation of this transporter is crucial for the regulation of nutrient uptake and, recently, a direct effect of both external and internal potassium and pH on ethanol tolerance in conditions relevant for the industrial production of bioethanol has been reported. In addition, several studies clearly indicate that the proteins involved in determining and maintaining plasma membrane potential through the modulation of potassium homeostasis represent promising targets for complimentary antifungal treatments. Therefore, the identification of proteins that regulate the activity of this potassium transporter has potential applications in both agronomic, industrial and medical contexts.
Hernanz-Koers M, Gandía M, Garrigues S, Manzanares P, Yenush L, Orzaez D, Marcos JF (2018)FungalBraid: A GoldenBraid-based modular cloning platform for the assembly and exchange of DNA elements tailored to fungal synthetic biologyFungal Genet Biol;116:51-61
Primo C, Ferri-Blázquez A, Loewith R, Yenush L (2017)Reciprocal Regulation of Target of Rapamycin Complex 1 and Potassium Accumulation.The Journal of Biological Chemistry 292: 563-574
Taibi K, del Campo AD, Aguado A, Mulet JM (2016)Early establishment response of different Pinus nigra ssp salzmanii seed sources on contrasting environments: Implications for future reforestation programs and assisted population migration
Journal of Environmental Management 171: 184-194
Yenush L (2016)Potassium and Sodium Transport in Yeast
Advances in Experimental Medicine and Biology 892: 187-228
Petrezselyova S, Lopez-Malo M, Canadell D, Roque A, Serra-Cardona A, Marquea MC, Vilaprinyo E, Alves R, Yenush L, Arino J (2016)Regulation of the Na+/K+-ATPase Ena1 Expression by Calcineurin/Crz1 under High pH Stress: A Quantitative StudyPlos One 11: e0158424
Llopis-Torregrosa V, Ferri-Blazquez A, Adam-Artigues A, Deffontaines E, van Heusden GPH, Yenush L (2016)Regulation of the Yeast Hxt6 Hexose Transporter by the Rod1 alpha-Arrestin, the Snf1 Protein Kinase, and the Bmh2 14-3-3 Protein.The Journal of biological chemistry 291: 14973-14985
Marqués MC, Zamarbide-Forés S, Pedelini L, Llopis-Torregrosa V, Yenush L (2015)A functional Rim101 complex is required for proper accumulation of the Ena1 Na+-ATPase protein in response to salt stress in Saccharomyces cerevisiae
FEMS Yeast Research 15: fov017
Mattiazzi Ušaj M, Prelec M, Brložnik M, Primo C, Curk T, Ščančar J, Yenush L, Petrovič U (2015)Yeast Saccharomyces cerevisiae adiponectin receptor homolog Izh2 is involved in the regulation of zinc, phospholipid and pH homeostasis
Metallomics 7: 1338-1351
Bueso E, Muñoz-Bertomeu J, Campos F, Brunaud V, Martinez L, Sayas E, Ballester P, Yenush L, Serrano R (2014)ARABIDOPSIS THALIANA HOMEOBOX 25 uncovers a role for gibberellins in seed longevity
Plant Physiology 164: 999-1010
Ariño J, Aydar E, Drulhe S, Ganser D, Jorrín J, Kahm M, Krause F, Petrezsélyová S, Yenush L, Zimmermannová O, van Heusden GP, Kschischo M, Ludwig J, Palmer C, Ramos J, Sychrová H (2014)Systems biology of monovalent cation homeostasis in yeast: the translucent contribution
Advances in Microbial Physiology 64: 1-63