Rules
High-quality RNA starting material is essential to successful microarray experimentation.
500 ng of total RNA is needed for Agilent Gene Expression microarrays.
RNA should be intact.
There should be no genomic DNA in the sample.
Samples should have an A260/A280 ratio of at least 1.8.
Min volume of the RNA sample is 5 µl, pref. concentration 100–150 ng/µl
RNA should be dissolved in aqueous solution (preferably DNase + RNase free water, not in DEPC water)