Research groups

Grupo: Plants Genomics and Biotechnology

Our research group is interested in the design of innovative agricultural goods using genomics, biotechnology and synthetic biology tools

Our main experimental system is the tomato fruit, and our aim is to increase its added value with new shapes, colors, aromas and healthy properties. For this, we browse the natural variability of tomato-related wild species in order to identify loci conferring fruit quality traits. Once those loci are identified, we use marker-assisted breeding to transfer them to modern tomato cultivars. Moving beyond the limits imposed by sexual hybridization and natural variability, we also make use of biotechnology tools as multigene engineering and genetic transformation to bring new quality traits into the tomato genome.

In a second line of research, we employ Synthetic Biology tools and principles for engineering plant biofactories of added-value products. In our lab, we have learnt how to build increasingly complex genetic instructions at the DNA level. By introducing these instructions into the genome of solanaceae species as tomato, tobacco and especially Nicotiana benthamiana (a tobacco relative), our goal is to re-direct the genetic program of the plant towards the synthesis of added-value products. Assisted by Agrobacterium tumefaciens and sometimes by viral vectors, we learn how to produce high levels of fine chemicals as antibodies, lectins, or snake anti-venoms in a short period of time..

For more information, visit our group webpage at http://www.ibmcp.upv.es/FGB/

RESEARCH INTERESTS

  • Molecular Breeding
  • Genetic Engineering and Synthetic Biology
  • Fruit Metabolomics
  • Molecular farming
Genómica y Biotecnología del fruto

Staff Researchers

Other Group Members

Technicians

  • Bernabé-Orts JM, Casas-Rodrigo I, Minguet EG, Landolfi V, Garcia-Carpintero V, Gianoglio S, Vázquez-Vilar M, Granell A, Orzaez D (2019)
    Assessment of Cas12a-mediated gene editing efficiency in plants
    Plant Biotechnol J. 10:1971-1984
  • Aliaga-Franco N, Zhang C, Presa S, Srivastava AK, Granell A, Alabadí D, Sadanandom A, Blázquez MA, Minguet EG (2019)
    Identification of Transgene-Free CRISPR-Edited Plants of Rice, Tomato, and Arabidopsis by Monitoring DsRED Fluorescence in Dry Seeds
    Front Plant Sci 10:1150
  • Selma S, Bernabé-Orts JM, Vazquez-Vilar M, Diego-Martin B, Ajenjo M, Garcia-Carpintero V, Granell A, Orzaez D (2019)
    Strong gene activation in plants with genome-wide specificity using a new orthogonal CRISPR/Cas9-based programmable transcriptional activator
    Plant Biotechnol J. 9:1703-1705
  • González-Mas MC, Rambla JL, López-Gresa MP, Blázquez MA, Granell A. (2019)
    Volatile Compounds in Citrus Essential Oils: A Comprehensive Review
    Front Plant Sci 10:12
  • Vazquez-Vilar M, Orzaez D, Patron N. (2018)
    DNA assembly standards: Setting the low-level programming code for plant biotechnology.
    Plant Sci. 273:33-41
  • Hamza R, Pérez-Hedo M, Urbaneja A, Rambla JL, Granell A, Gaddour K, Beltrán JP, Cañas LA (2018)
    Expression of two barley proteinase inhibitors in tomato promotes endogenous defensive response and enhances resistance to Tuta absoluta
    BMC Plant Biol. 18(1):24
  • Esteras C, Rambla JL, Sánchez G, López-Gresa MP, González-Mas MC, Fernández-Trujillo JP, Bellés JM, Granell A, Picó MB (2018)
    Fruit flesh volatile and carotenoid profile analysis within the Cucumis melo L. species reveals unexploited variability for future genetic breeding
    J Sci Food Agric.98(10):3915-3925
  • Hernanz-Koers M, Gandía M, Garrigues S, Manzanares P, Yenush L, Orzaez D, Marcos JF (2018)
    FungalBraid: A GoldenBraid-based modular cloning platform for the assembly and exchange of DNA elements tailored to fungal synthetic biology
    Fungal Genet Biol;116:51-61
  • Rubio-Cabetas MJ, Pons C, Bielsa B, Amador ML, Marti C, Granell A (2018)
    Preformed and induced mechanisms underlies the differential responses of Prunus rootstock to hypoxia
    J Plant Physiol. 228:134-149
  • Tieman D, Zhu G, Resende MF Jr, Lin T, Nguyen C, Bies D, Rambla JL, Beltran KS, Taylor M, Zhang B, Ikeda H, Liu Z, Fisher J, Zemach I, Monforte A, Zamir D, Granell A, Kirst M, Huang S, Klee H (2017)
    A chemical genetic roadmap to improved tomato flavor
    Science 355(6323):391-394
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SUSPHIRE: Sustainable Bioproduction of Pheromones for Insect Pest Control in Agriculture.

Project Start: June 2018. Project end: May 2021. Duration: 36 months

Project Website: http://susphire.info

 

Entidad financiadora: 
ERA CoBioTech partners: BMBF, MIZS, AEI, CDTI, UKRI-BBSRC, co-funded by the European Union.
IP: 
Dr Diego Orzáez (Project Coordinator)

NEWCOTIANA: Developing Multipurpose Nicotiana Crops for Molecular Farming using New Plant Breeding Techniques.

Project Start: January 2018. Project end: June 2022. Duration: 54 months

Project Website: http://newcotiana.org

 

Entidad financiadora: 
European Union’s Horizon 2020 Research and Innovation programme (H2020-NMBP-BIO-2017)
IP: 
Dr Diego Orzaez (Project Coordinator)

Pharma-Factory: Delivering new and innovative high value products using plant molecular farming.

Project start: November 2017. Project end: October 2021. Duration: 48 months

Project Website: https://pharmafactory.org

 

Entidad financiadora: 
EU (H2020-BB-2017-1; topic:BB-07-2017 - Plant Molecular Factory)
IP: 
IP CSIC: Dr Diego Orzaez; Project Coordinator:Julian Ma

Design of synthetic orthogonal regulatory gene circuits in plants using CRISPR-Cas9-like programmable DNA-binding proteins..

Project start: January 2017. Project end: December 2019. Duration: 36 months

 

Entidad financiadora: 
Spanish Ministry of Economy and Competitiveness (BIO2016-78601-R). Co-financed by FEDER.
IP: 
Dr Diego Orzaez, Prof Antonio Granell

TomGEM: A holistic multi-actor approach towards the design of new tomato varieties and management practices to improve yield and quality in the face of climate change.

Project start: March 2016. Project end: February 2020. Duration: 48 months

Project Website: https://tomgem.eu/

 

Entidad financiadora: 
EC H2020 topic SFT-05-2015
IP: 
IP CSIC: Prof Antonio Granell; Project Coordinator: Prof. Mondher Bouzayen

Nuevos productos cosméticos y sanitarios mediante producción recombinante de proteínas y anticuerpos para inmunoafinidad.

Project start: March 2015. Project end: June 2017. Duration: 28 months

 

Entidad financiadora: 
Spanish Ministry of Economy and Competitiveness (RTC-2015-4374-1). Co-financed by FEDER.
IP: 
Dr Diego Orzaez

TRADITOM. Traditional tomato varieties and cultural practicies: a case for agricultural diversification with impact on food security and health of European population.

Project start: March 2015. Project end: October 2018. Duration: 42 months

Project Website: http://www.traditom.eu/

 

Entidad financiadora: 
European Union’s Horizon 2020 Research and Innovation programme. Topic SFS-7a-2014
IP: 
Prof Antonio Granell (Project Coordinator)

Green switches: Design of synthetic gene circuits for recombinant protein production and nutritional enhancement in Solanaceous plants.

Project start: January 2014. Project end: December 2016. Duration: 36 months

 

Entidad financiadora: 
Spanish Ministry of Economy and Competitiveness (BIO2013-42193-R). Co-financed by BEI and FEDER.
IP: 
Dr Diego Orzaez, Prof Antonio Granell

Production of recombinant polyclonal antibodies in Nicotiana benthamiana plants in an experimental greenhouse.

Project start: January 2014. Project end: OCtober 2014. Duation: 10 months

 

Entidad financiadora: 
Polytechnic University Valencia; Programa de Valorización VLC/CAMPUS: Prueba de Concepto (PdC13)
IP: 
Dr Diego Orzaez

Production of Therapeutic Proteins in Plant Biofactories - PROTEBIOV.

Project start: July 2011. Duration: 42 months

 

Entidad financiadora: 
INNPACTO, Spanish Ministry of Science and Innovation. Co-financed by FEDER.
IP: 
IP CSIC: Dr Diego Orzaez. Project Coordinator: IDEN BIOTECHNOLOGY S.L.

Engineering Tomatoes for Health: Building Blocks for Intragenesis and Molecular Farming in Solanaceae.

Project start: January 2011. Project end: May 2013. Duration: 36 months

 

Entidad financiadora: 
Spanish Ministry of Science and Innovation (BIO2010-15384)
IP: 
Dr Diego Orzaez
Título: 
No hay datos sobre las patentes del grupo
Development and characterization of two new tools for plant genetic engineering: A CRISPR/Cas12a-based mutagenesis system and a PhiC31-based gene switch
Año: 
2019
Nombre: 
Joan Miquel Bernabé Orts
Universidad: 
UPV
Dirigida por: 
Dr. Diego Orzáez Calatayud
Tipo: 
Tesis Doctorales
Desarrollo de activadores transcripcionales programables para plantas basados en el sistema CRISPR/Cas9
Año: 
2018
Nombre: 
Borja Diego Martin
Universidad: 
UPV/EHU
Dirigida por: 
Dr Diego Orzáez Calatayud, Sara Selma
Tipo: 
Trabajos de Fin de Carrera
Estudio de la conmutación de un interruptor genético basado en la integrasa del fago PhiC31 en Nicotiana benthamiana
Año: 
2018
Nombre: 
Javier Macheño Bonillo
Universidad: 
UPV
Dirigida por: 
Dr Diego Orzáez Calatayud
Tipo: 
Trabajos de Fin de Carrera
Implementation of CRISPR-based gene targeting tools for genome engineering in Plant Synthetic Biology
Año: 
2018
Nombre: 
Alicia Climent Catalá
Universidad: 
UPV
Dirigida por: 
Dr Diego Orzáez Calatayud, Dr Marta Vazquez Vilar
Tipo: 
Trabajos de Fin de Carrera
Estudio de la regulación transcripcional del promotor de la proteína metalotioneína de tipo 2B mediante el sistema CRISPR/Cas9 en Nicotiana benthamiana
Año: 
2018
Nombre: 
María Ajenjo Bauzá
Universidad: 
UPV
Dirigida por: 
Dr Diego Orzáez Calatayud, Sara Selma
Tipo: 
Trabajos de Fin de Carrera
Diseño de un dispositivo genético para la determinación de la eficiencia de edición de genes mediada por CRISPR en plantas
Año: 
2018
Nombre: 
Agostina De Luca Pauluzzi
Universidad: 
UPV
Dirigida por: 
Dr. Diego Orzáez Calatayud, Joan Miquel Bernabé Orts, Dr Marta Vázquez Vilar
Tipo: 
Trabajos de Fin de Carrera
A multilevel, developmentally controlled gene engineering strategy for tomato fortification and protection
Año: 
2017
Nombre: 
María Florencia Cocaliadis Caisson
Universidad: 
UPV
Dirigida por: 
Prof Antonio Granell Richart / Dr Diego Orzáez Calatayud
Tipo: 
Tesis Doctorales
New methodologies for the production of recombinant antibodies in plants
Año: 
2017
Nombre: 
Estefania Huet Trujillo
Universidad: 
UPV
Dirigida por: 
Dr Diego Orzáez Calatayud
Tipo: 
Tesis Doctorales
Implementación y caracterización de nuevas versiones del sistema CRISPR para la Biología Sintética de Plantas
Año: 
2017
Nombre: 
Iván Casas Rodrigo
Universidad: 
UPV
Dirigida por: 
Dr Diego Orzáez Calatayud
Tipo: 
Trabajos de Fin de Carrera
Design of genetic elements and software tools for plant synthetic biology
Año: 
2016
Nombre: 
Marta Vázquez Vilar
Universidad: 
UPV
Dirigida por: 
Dr Diego Orzáez Calatayud
Tipo: 
Tesis Doctorales