Plants host a large variety of infectious agents that frequently cause enormous damage in crops and natural ecosystems. The first goal of our research group is to understand the molecular mechanisms that underlie the interaction between plants and some of these pathogens, such as viruses and viroids. From this knowledge we expect to develop new biotechnological strategies for crop protection and innovation. In addition, we believe that we can take advantage of the remarkable biological properties of plant viruses and viroids by converting them into useful biotechnological devices. Our second goal is to develop systems to produce products of interest (metabolites, RNAs, proteins, antibodies...) in biofactory plants using conveniently engineered viruses and viroids. We envision a future in which cultivated plants will be the most reliable and sustainable source of food, feed, fibers, fuel, and pharmaceutical and chemical products for human kind.
More specifically, some of the ongoing projects in our research group aim to:
1. Decipher how viroid RNAs are able to replicate in plant cells. See Cordero et al. 2018, Front. Microbiol.
2. Understand the plant defensive response to virus infection. See Cordero et al. 2017, Mol. Plant Microbe Interact.
3. Identify resistance genes to virus infection. See Aragonés et al. 2018, Eur. J. Plant Pathol.
4. Develop biotechnological strategies to protect plants against virus and viroids. See Carbonell et al. 2017, Mol. Plant Pathol.; or Carbonell et al. 2018, Mol. Plant Microbe Interact.
5. Produce health promoting carotenoids and anthocyanins in plants using viral vectors. See Majer et al. 2017, Sci. Rep.; or Cordero et al. 2017, Front. Microbiol.
6. Produce large amounts of recombinant RNAs using a viroid-based vector system. See Daròs et al., Sci. Rep.
7. Develop viral vectors to produce recombinant proteins in plants. See Majer et al. 2015, Biotechnol. J.; or Shi et al. 2018, Plant Biotech. J.
8. Build synthetic gene circuits to regulate gene expression in plants. See Cordero et al. 2018, ACS Synth. Biol.
Other Group Members
Carbonell, A (2019)Design and high-throughput generation of artificial small RNA constructs for plants. In: Plant MicroRNAs: Methods and ProtocolsMethods in Molecular Biology 1932: 247-260
Carbonell, A. and Daròs, J.A (2019)Design, Synthesis, and Functional Analysis of Highly Specific Artificial Small RNAs With Antiviral Activity in Plants. In: Antiviral Resistance in Plants: Methods and ProtocolsMethods in Molecular Biology 2028: 231-246
Carbonell, A., López, C. and Daròs, J.A (2019)Fast-forward identification of highly effective artificial small RNAs against different Tomato spotted wilt virus isolatesMolecular Plant-Microbe Interactions 32 (2): 142-156
Luis Cervera-Seco, María Carmen Marqués, Alejandro Sanz-Carbonell, Joan Márquez-Molins, Alberto Carbonell, José-Antonio Daròs and Gustavo Gómez (2019)Identification and characterization of a stress-responsive TAS3-derived tasiRNA in melonPlant and Cell Physiology 60 (11): 2382-2393
Carbonell, A., Purificación Lisón and Daròs, J.A (2019)Multi-targeting of viral RNAs with synthetic trans-acting small interfering RNAs enhances plant antiviral resistanceThe Plant Journal 100: 720-737
Carbonell, A (2019)Secondary small interfering RNA-based silencing tools in plants: an updateFrontiers in Plant Science 10: 687
Carbonell, A (2017)Immunoprecipitation and high-throughput sequencing of ARGONAUTE-bound RNAs from plants. In: Plant Argonaute Proteins: Methods and ProtocolsMethods in Molecular Biology 1640: 93-112
Kénesi, E., Carbonell, A., Lózsa, R., Vértessy, B. and Lakatos, L (2017)A viral suppressor of RNA silencing inhibits ARGONAUTE 1 function by precluding target RNA binding to pre-assembled RISCNucleic Acids Research 45 (13): 7736-7750
Carbonell, A. and Daròs, J.A (2017)Artificial microRNAs and synthetic trans-acting small interfering RNAs interfere with viroid infectionMolecular Plant Pathology 18 (5): 746-753
Carbonell, A (2017)Artificial small RNA-based strategies for effective and specific gene silencing in plantsPlant Gene Silencing: Mechanisms and Applications 6: 110-127.