Research groups

Grupo: Citrus Biotechnology

CITRUS BIOTECHNOLOGY

Our laboratory is focused on using biotechnology to address important challenges of interest for the citrus industry, including the incessant demand of the market to generate citrus types resistant to emerging diseases that are ravaging the world citrus industry such as Huanglongbing (HLB) or citrus black spot. Another research line of the group is based on increasing the content of specific micronutrients or healthy phytochemical compounds in citrus fruits, in order to improve their nutri-functional properties. We, therefore, intend to develop new varieties that meet the current needs of the citrus industry, in addition to serving as tools to broaden our understanding of the role of terpene volatiles, flavonoids and carotenoids from fruits in their interactions with the biotic environment. Another recent line of research is to determine the role of the terpenes present in orange peel in the interaction of citrus fruits with frugivorous animals, and their consequences on citrus seed dispersal in natural environments. The main research lines of our group are:

• Generating varieties repellent/resistant to major insect vectors and pathogens affecting citrus such as Diaphorina citri (vector of the bacterium that causes HLB), Phyllosticta citricarpa (which causes citrus black spot) or Xanthomonas citri subsp. citri (causing citrus canker), among others.

• Bring an added value to citrus fruits by increasing their content in healthy compounds such as specific carotenoids and anthocyanins, as well as cloning and characterizing specific fruit promoters from citrus for biotechnological use.

• Modulation of vegetative development of citrus varieties by using dwarfing rootstocks and induction of early flowering/fruiting in juvenile plants as a tool for rapid analysis of flower/fruit improvement traits.

• Studies on the consumption of fruits by animals, seed dispersal and possible naturalization of transgenic citrus trees under field conditions.

Staff Researchers

Other Group Members

Temporary Visitors

Stefania Benici (estudiante en estancia)
  • Rodrigo, MJ; Lado, J., Alós, E., Alquézar, B., Dery, O., Hirschberg, J., Zacarías, L (2019)
    A mutant allele of ζ-carotene isomerase (ZISO) is associated with the yellow pigmentation of the “Pinalate” sweet orange mutant and reveals new insights into its role in fruit carotenogenesis
    BMC Plant Biology 19:465
  • Ruiz-Ruiz, S., Navarro, B., PEÑA, L., Navarro, L., Moreno, P., Di Serio, F., Flores, R (2019)
    Citrus Tristeza Virus: Host RNA Silencing and Virus Counteraction. Citrus Tristeza Virus: Methods and Protocols
    Methods in Molecular Biology 2015: 195-207
  • Soler, N., Plomer, M., Fagoaga, C., Moreno, P., Navarro, L., Flores, R. and PEÑA, L (2019)
    Methods for producing transgenic plants resistant to CTV. Citrus Tristeza Virus: Methods and Protocols
    Methods in Molecular Biology 2015: 229-243
  • Cifuentes-Arenas, J.C., Beattie, G.A.C., Peña, L., Lopes, S.A (2019)
    Murraya paniculata and Swinglea glutinosa as short-term transient hosts of ‘Candidatus Liberibacter asiaticus’ and implications for spread of Huanglongbing.
    Phytopathology
  • Caland, R.B.O., Cadavid, C.O.M., Carmona, L., PEÑA, L., Oliveira, R.P (2019)
    Pasteurized orange juice rich in carotenoids protects Caenorhabditis elegans against oxidative stress and β-amyloid toxicity through direct and indirect mechanisms
    Oxidative Medicine and Cellular Longevity. Article ID 5046280,
  • Carmona, L., Alquézar, B., Tárraga, S., PEÑA, L (2019)
    Protein analysis of Moro blood orange pulp during storage at low temperatures
    Food Chemistry 277: 75-83
  • Peris J.E., Malara T., Borges R., Falconi J.R., Peña, L., Fedriani, J.M. (2019)
    Reunion in the overseas: introduced wild boars and cultivated orange trees interact in the Mata Atlântica (Brazil).
    International Journal of Tropical Biology 67 (4): 901-912
  • Felisberto, P.A.C, Girardi, E.A., PEÑA, L. Felisberto, G., Beattie, G.A.C., Lopes (2019)
    Unsuitability of indigenous South American Rutaceae as potential hosts of Diaphorina citri
    Pest Management Science, DOI 10.1002/ps.5304
  • Wulff, Nelson A., Marques, Viviani V., Peris, Josep Enric., Pons, Elsa, Sanches, Andre L., Peña, Leandro (2018)
    Biossegurança de árvores frutíferas geneticamente modificadas no campo: o caso dos cítricos
    Biossegurança de árvores. Capítulo 12
  • Ruiz-Ruiz, S., Spàno, R., Navarro, L., Moreno, P., PEÑA, L. and Flores, R (2018)
    Citrus tristeza virus co-opts glyceraldehyde 3-phosphate dehydrogenase for its infectious cycle by interacting with the viral-encoded protein p24

    Plant Molecular Biology

Tomaseto, A.F., Marques, R.N., Fereres, A., Zanardi, O.Z., Volpe, H.X.L., Alquézar, B., PEÑA, L. and Miranda, M.P. (2019)
Orange jasmine as a trap crop to control Diaphorina citri.
Scientific Reports 9: 2070 | https://doi.org/10.1038/s41598-019-38597-5, 2019
Soler, N., Plomer, M., Fagoaga, C., Moreno, P., Navarro, L., Flores, R. and PEÑA, L. (2019)
Methods for producing transgenic plants resistant to CTV.
Methods for producing transgenic plants resistant to CTV. Citrus Tristeza Virus: Methods and Protocols (Catara, A, Bar-Joseph, J and Licciardello, G., ed.) Methods in Molecular Biology, in press, 2019.
Felisberto, P.A.C, Girardi, E.A., PEÑA, L. Felisberto, G., Beattie, G.A.C. and Lopes, S.A. (2019)
Unsuitability of indigenous South American Rutaceae as potential hosts of Diaphorina citri.
Pest Management Science, DOI 10.1002/ps.5304, 2019.
Caland, R.B.O., Cadavid, C.O.M., Carmona, L., PEÑA, L. and Oliveira, R.P. (2019)
Pasteurized orange juice rich in carotenoids protects Caenorhabditis elegans against oxidative stress and β-amyloid toxicity through direct and indirect mechanisms
Oxidative Medicine and Cellular Longevity, in press, 2019.
Ruiz-Ruiz, S., Navarro, B., PEÑA, L., Navarro, L., Moreno, P., Di Serio, F. and Flores, R. (2019)
Citrus Tristeza Virus: Host RNA Silencing and Virus Counteraction
Citrus Tristeza Virus: Host RNA Silencing and Virus Counteraction. Citrus Tristeza Virus: Methods and Protocols (Catara, A, Bar-Joseph, J and Licciardello, G., ed.) Methods in Molecular Biology, in press, 2019.
Rodríguez, Ana; Kava, Vanessa; Latorre-García, Lorena; da Silva jr., Geraldo J.; Pereira, Rosana G.; Glienke, Chirlei; Ferreira-Maba, Lisandra S.; Vicent, Antonio; Shimada, Takehiko; Peña, Leandro (2018)
Engineering D-limonene synthase down-regulation in orange fruit induces resistance against the fungus Phyllosticta citricarpa through enhanced accumulation of monoterpene alcohols and activation of defence.
Molecular Plant Pathology. 19 - 9, pp. 2077 - 2093. 2018.
Ruiz-Ruiz, S., Spàno, R., Navarro, L., Moreno, P., PEÑA, L. and Flores, R. (2018)
Citrus tristeza virus co-opts glyceraldehyde 3-phosphate dehydrogenase for its infectious cycle by interacting with the viral-encoded protein p23.
Plant Molecular Biology 98: 363-373, 2018. DOI: 10.1007/s11103-018-0783-0.
Carmona, L., Alquézar, B., Tárraga, S. and PEÑA, L. (2018)
Protein analysis of Moro blood orange pulp during storage at low temperatures
Food Chemistry 277: 75-83, 2019. DOI: 10.1016/j.foodchem.2018.10.108
Soler, N., Plomer, M., Fagoaga, C., Moreno, P., Navarro, L., Flores, R. and PEÑA, L. Methods for producing transgenic plants resistant to CTV. Methods in Molecular Biology, in press, 2018. (2018)
Methods for producing transgenic plants resistant to CTV.
Methods in Molecular Biology, in press, 2018.
Wulff, Nelson A., Marques, Viviani V., Peris, Josep Enric., Pons, Elsa, Sanches, Andre L., Peña, Leandro (2018)
Biossegurança de árvores frutíferas geneticamente modificadas no campo: o caso dos cítricos.
Biossegurança de árvores. Capítulo 12. E-Book. (Pasquali, G., Ed.), in press, 2018.
No hay información sobre proyectos
Entidad financiadora: 
FAPESP
IP: 
LEANDRO PEÑA.
PRE-HLB: PREVENTING HLB EPIDEMICS FOR ENSURING CITRUS SURVIVAL IN EUROPE Organismo Financiador: EU H2020-SFS-2018-2 Entidades participantes: CSIC y otras 23 entidades de 9 países Coordinador General (e IP de un WP): LEANDRO PEÑA. Fechas: 2019- 2023. 8.001.690 € (6.639.815 € financiados por UE)
Entidad financiadora: 
EU H2020-SFS-2018-2
IP: 
LEANDRO PEÑA.
Título: 
Repellent compositions and genetic approaches for controlling Huanglongbing.
Referencia ID: 
Application No. 14/733,439; Filed: June 8, 2015 Continuation-in-part of U.S. Application No. 13/501,739 filed April 12, 2012. Patent No. US 9,572,349 B2; Patent Date: February 21, 2017.
Autores: 
Alquézar, Berta, Rodríguez, Ana, Peris, Josep, Peña, Leandro, Henrique, Andreia, Pedreira, Marcelo, Yamamoto, Pedro T., Sposito, Marcelo B., Wulff, Nelson A., Teixeira, D.C., Ayres, A. Juliano, Cavalcanti, Newton, Simoes Bento, J. Mauricio, Parra, Jose Roberto P.
Título: 
Repellent compositions and genetic approaches for controlling Huanglongbing
Referencia ID: 
U.S.A. Patent No. 9,078,448, which is the US national phase of PCT application PCT/BR2010/000353. Date of patent: Oct. 28, 2009. PCT WO2011/050432 A1. Fundecitrus/IVIA. Patente aceptada (Enero de 2015) en China, Unión Europea, Sudáfrica y Chile
Autores: 
Alquézar, Berta, Rodríguez, Ana, Peris, Josep, Peña, Leandro, Henrique, Andreia, Pedreira, Marcelo, Yamamoto, Pedro T., Sposito, Marcelo B., Wulff, Nelson A., Teixeira, D.C., Ayres, A. Juliano, Cavalcanti, Newton, Simoes Bento, J. Mauricio, Parra, Jose Roberto P.
Título: 
Método para conseguir resistencia frente a enfermedades de los cítricos causadas por insectos, por hongos u omicetos o por bacterias o nematodos.
Referencia ID: 
2007. Solicitud Patente de Invención nº 200901283
Autores: 
Rodríguez, Ana; Cervera, Magdalena; Shimada, Takehiko; Redondo, Ana; Rodrigo, M. Jesús; San Andrés, Victoria; Palou, Lluís; Zacarías, Lorenzo; López, M. Milagros; Castañera, Pedro and Peña, Leandro.
Título: 
Method for generating resistance against citrus diseases caused by insects, fungi, oomycetes, bacteria or nematodes.
Referencia ID: 
2010. Patent number: 12/945,972
Autores: 
Rodríguez, Ana; Cervera, Magdalena; Shimada, Takehiko; Redondo, Ana; Rodrigo, M. Jesús; San Andrés, Victoria; Palou, Lluís; Zacarías, Lorenzo; López, M. Milagros; Castañera, Pedro and Peña, Leandro.